benzo a pyrene Search Results


benzo a pyrene bap  (LGC Standards)
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6 chloro benzo a pyrene  (ZeptoMetrix corporation)
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carcinogenic agent benzo  (Santa Cruz Biotechnology)
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bap  (Santa Cruz Biotechnology)
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benzo a pyrene diol epoxide bpde  (Santa Cruz Biotechnology)
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FIGURE 1 | Effects of S- and R-carvedilol on <t>BPDE-induced</t> lung epithelial cell transformation: Benzo(a)pyrene diol epoxide (BPDE), an active metabolite of B(a)P, was used to transform BEAS-2B cells. (A) & (B) The cells were pre-treated with 5 μM S- and R-carvedilol, 5 μM atenolol, 5 μM pro- pranolol (Prop), and 5 μM ICI-118551 (ICI) for 2 h, and then treated with 0.2 μM of BPDE for 1 h. The cells were then cultured with 5 μM of the drugs for 7 days. Afterward, they were seeded in soft agar in a 96-well plate (2000 cells/well) containing 5 μM of the drugs in the top layer of agar. The cell colonies were counted under a microscope after 10 days of incubation. (C) Representative images were taken using GelCount of the wells containing colonies that grew on agar after 10 days of incubation. The plotted data are represented as mean ± SD, n = 6 to 8. The one-way ANOVA followed by Dunnett's multiple comparison test was used to assess statistical differences. Statistical significance was denoted as ****: P < 0.0001 and **: P < 0.01.
Benzo A Pyrene Diol Epoxide Bpde, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trans 7 8 dihydroxy 7 8 dihydro b  (Toronto Research Chemicals)
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FIGURE 1 | Effects of S- and R-carvedilol on <t>BPDE-induced</t> lung epithelial cell transformation: Benzo(a)pyrene diol epoxide (BPDE), an active metabolite of B(a)P, was used to transform BEAS-2B cells. (A) & (B) The cells were pre-treated with 5 μM S- and R-carvedilol, 5 μM atenolol, 5 μM pro- pranolol (Prop), and 5 μM ICI-118551 (ICI) for 2 h, and then treated with 0.2 μM of BPDE for 1 h. The cells were then cultured with 5 μM of the drugs for 7 days. Afterward, they were seeded in soft agar in a 96-well plate (2000 cells/well) containing 5 μM of the drugs in the top layer of agar. The cell colonies were counted under a microscope after 10 days of incubation. (C) Representative images were taken using GelCount of the wells containing colonies that grew on agar after 10 days of incubation. The plotted data are represented as mean ± SD, n = 6 to 8. The one-way ANOVA followed by Dunnett's multiple comparison test was used to assess statistical differences. Statistical significance was denoted as ****: P < 0.0001 and **: P < 0.01.
Trans 7 8 Dihydroxy 7 8 Dihydro B, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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benzo a pyrene 3 6 quinone bpq  (Toronto Research Chemicals)
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FIGURE 1 | Effects of S- and R-carvedilol on <t>BPDE-induced</t> lung epithelial cell transformation: Benzo(a)pyrene diol epoxide (BPDE), an active metabolite of B(a)P, was used to transform BEAS-2B cells. (A) & (B) The cells were pre-treated with 5 μM S- and R-carvedilol, 5 μM atenolol, 5 μM pro- pranolol (Prop), and 5 μM ICI-118551 (ICI) for 2 h, and then treated with 0.2 μM of BPDE for 1 h. The cells were then cultured with 5 μM of the drugs for 7 days. Afterward, they were seeded in soft agar in a 96-well plate (2000 cells/well) containing 5 μM of the drugs in the top layer of agar. The cell colonies were counted under a microscope after 10 days of incubation. (C) Representative images were taken using GelCount of the wells containing colonies that grew on agar after 10 days of incubation. The plotted data are represented as mean ± SD, n = 6 to 8. The one-way ANOVA followed by Dunnett's multiple comparison test was used to assess statistical differences. Statistical significance was denoted as ****: P < 0.0001 and **: P < 0.01.
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standards  (LGC Standards)
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LGC Standards standards
FIGURE 1 | Effects of S- and R-carvedilol on <t>BPDE-induced</t> lung epithelial cell transformation: Benzo(a)pyrene diol epoxide (BPDE), an active metabolite of B(a)P, was used to transform BEAS-2B cells. (A) & (B) The cells were pre-treated with 5 μM S- and R-carvedilol, 5 μM atenolol, 5 μM pro- pranolol (Prop), and 5 μM ICI-118551 (ICI) for 2 h, and then treated with 0.2 μM of BPDE for 1 h. The cells were then cultured with 5 μM of the drugs for 7 days. Afterward, they were seeded in soft agar in a 96-well plate (2000 cells/well) containing 5 μM of the drugs in the top layer of agar. The cell colonies were counted under a microscope after 10 days of incubation. (C) Representative images were taken using GelCount of the wells containing colonies that grew on agar after 10 days of incubation. The plotted data are represented as mean ± SD, n = 6 to 8. The one-way ANOVA followed by Dunnett's multiple comparison test was used to assess statistical differences. Statistical significance was denoted as ****: P < 0.0001 and **: P < 0.01.
Standards, supplied by LGC Standards, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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benzo a pyrene  (LGC Standards)
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FIGURE 1 | Effects of S- and R-carvedilol on <t>BPDE-induced</t> lung epithelial cell transformation: Benzo(a)pyrene diol epoxide (BPDE), an active metabolite of B(a)P, was used to transform BEAS-2B cells. (A) & (B) The cells were pre-treated with 5 μM S- and R-carvedilol, 5 μM atenolol, 5 μM pro- pranolol (Prop), and 5 μM ICI-118551 (ICI) for 2 h, and then treated with 0.2 μM of BPDE for 1 h. The cells were then cultured with 5 μM of the drugs for 7 days. Afterward, they were seeded in soft agar in a 96-well plate (2000 cells/well) containing 5 μM of the drugs in the top layer of agar. The cell colonies were counted under a microscope after 10 days of incubation. (C) Representative images were taken using GelCount of the wells containing colonies that grew on agar after 10 days of incubation. The plotted data are represented as mean ± SD, n = 6 to 8. The one-way ANOVA followed by Dunnett's multiple comparison test was used to assess statistical differences. Statistical significance was denoted as ****: P < 0.0001 and **: P < 0.01.
Benzo A Pyrene, supplied by LGC Standards, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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benzo a pyrene d12  (LGC Standards)
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FIGURE 1 | Effects of S- and R-carvedilol on <t>BPDE-induced</t> lung epithelial cell transformation: Benzo(a)pyrene diol epoxide (BPDE), an active metabolite of B(a)P, was used to transform BEAS-2B cells. (A) & (B) The cells were pre-treated with 5 μM S- and R-carvedilol, 5 μM atenolol, 5 μM pro- pranolol (Prop), and 5 μM ICI-118551 (ICI) for 2 h, and then treated with 0.2 μM of BPDE for 1 h. The cells were then cultured with 5 μM of the drugs for 7 days. Afterward, they were seeded in soft agar in a 96-well plate (2000 cells/well) containing 5 μM of the drugs in the top layer of agar. The cell colonies were counted under a microscope after 10 days of incubation. (C) Representative images were taken using GelCount of the wells containing colonies that grew on agar after 10 days of incubation. The plotted data are represented as mean ± SD, n = 6 to 8. The one-way ANOVA followed by Dunnett's multiple comparison test was used to assess statistical differences. Statistical significance was denoted as ****: P < 0.0001 and **: P < 0.01.
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benzo  (LKT Laboratories)
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FIGURE 1 | Effects of S- and R-carvedilol on <t>BPDE-induced</t> lung epithelial cell transformation: Benzo(a)pyrene diol epoxide (BPDE), an active metabolite of B(a)P, was used to transform BEAS-2B cells. (A) & (B) The cells were pre-treated with 5 μM S- and R-carvedilol, 5 μM atenolol, 5 μM pro- pranolol (Prop), and 5 μM ICI-118551 (ICI) for 2 h, and then treated with 0.2 μM of BPDE for 1 h. The cells were then cultured with 5 μM of the drugs for 7 days. Afterward, they were seeded in soft agar in a 96-well plate (2000 cells/well) containing 5 μM of the drugs in the top layer of agar. The cell colonies were counted under a microscope after 10 days of incubation. (C) Representative images were taken using GelCount of the wells containing colonies that grew on agar after 10 days of incubation. The plotted data are represented as mean ± SD, n = 6 to 8. The one-way ANOVA followed by Dunnett's multiple comparison test was used to assess statistical differences. Statistical significance was denoted as ****: P < 0.0001 and **: P < 0.01.
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d-sorbitol method  (FUJIFILM)
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FIGURE 1 | Effects of S- and R-carvedilol on <t>BPDE-induced</t> lung epithelial cell transformation: Benzo(a)pyrene diol epoxide (BPDE), an active metabolite of B(a)P, was used to transform BEAS-2B cells. (A) & (B) The cells were pre-treated with 5 μM S- and R-carvedilol, 5 μM atenolol, 5 μM pro- pranolol (Prop), and 5 μM ICI-118551 (ICI) for 2 h, and then treated with 0.2 μM of BPDE for 1 h. The cells were then cultured with 5 μM of the drugs for 7 days. Afterward, they were seeded in soft agar in a 96-well plate (2000 cells/well) containing 5 μM of the drugs in the top layer of agar. The cell colonies were counted under a microscope after 10 days of incubation. (C) Representative images were taken using GelCount of the wells containing colonies that grew on agar after 10 days of incubation. The plotted data are represented as mean ± SD, n = 6 to 8. The one-way ANOVA followed by Dunnett's multiple comparison test was used to assess statistical differences. Statistical significance was denoted as ****: P < 0.0001 and **: P < 0.01.
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Image Search Results


FIGURE 1 | Effects of S- and R-carvedilol on BPDE-induced lung epithelial cell transformation: Benzo(a)pyrene diol epoxide (BPDE), an active metabolite of B(a)P, was used to transform BEAS-2B cells. (A) & (B) The cells were pre-treated with 5 μM S- and R-carvedilol, 5 μM atenolol, 5 μM pro- pranolol (Prop), and 5 μM ICI-118551 (ICI) for 2 h, and then treated with 0.2 μM of BPDE for 1 h. The cells were then cultured with 5 μM of the drugs for 7 days. Afterward, they were seeded in soft agar in a 96-well plate (2000 cells/well) containing 5 μM of the drugs in the top layer of agar. The cell colonies were counted under a microscope after 10 days of incubation. (C) Representative images were taken using GelCount of the wells containing colonies that grew on agar after 10 days of incubation. The plotted data are represented as mean ± SD, n = 6 to 8. The one-way ANOVA followed by Dunnett's multiple comparison test was used to assess statistical differences. Statistical significance was denoted as ****: P < 0.0001 and **: P < 0.01.

Journal: Thoracic cancer

Article Title: S- and R-Carvedilol Prevent Benzo(a)pyrene-Induced Lung Carcinogenesis.

doi: 10.1111/1759-7714.70109

Figure Lengend Snippet: FIGURE 1 | Effects of S- and R-carvedilol on BPDE-induced lung epithelial cell transformation: Benzo(a)pyrene diol epoxide (BPDE), an active metabolite of B(a)P, was used to transform BEAS-2B cells. (A) & (B) The cells were pre-treated with 5 μM S- and R-carvedilol, 5 μM atenolol, 5 μM pro- pranolol (Prop), and 5 μM ICI-118551 (ICI) for 2 h, and then treated with 0.2 μM of BPDE for 1 h. The cells were then cultured with 5 μM of the drugs for 7 days. Afterward, they were seeded in soft agar in a 96-well plate (2000 cells/well) containing 5 μM of the drugs in the top layer of agar. The cell colonies were counted under a microscope after 10 days of incubation. (C) Representative images were taken using GelCount of the wells containing colonies that grew on agar after 10 days of incubation. The plotted data are represented as mean ± SD, n = 6 to 8. The one-way ANOVA followed by Dunnett's multiple comparison test was used to assess statistical differences. Statistical significance was denoted as ****: P < 0.0001 and **: P < 0.01.

Article Snippet: Benzo(a)pyrene was purchased from Sigma- Aldrich (St. Louis, MO), while Benzo(a)pyrene Diol Epoxide (BPDE) was obtained from Santa Cruz Biotechnology.

Techniques: Transformation Assay, Cell Culture, Microscopy, Incubation, Comparison